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Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Wertheim cung cấp cho các bạn kiến thức về ngành y đề tài: Ancient genomes. | Minireview Ancient genomes A Rus Hoelzel Address School of Biological and Biomedical Sciences Durham University South Road Durham DH1 3LE UK. E-mail a.r.hoelzel@dur.ac.uk Published I December 2005 Genome Biology 2005 6 239 doi I0.II86 gb-2005-6-I2-239 The electronic version of this article is the complete one and can be found online at http genomebiology.com 2005 6 I2 239 2005 BioMed Central Ltd Abstract Ever since its invention the polymerase chain reaction has been the method of choice for work with ancient DNA. In an application of modern genomic methods to material from the Pleistocene a recent study has instead undertaken to clone and sequence a portion of the ancient genome of the cave bear. Nearly 20 years ago a researcher from down the hall in the Department of Genetics at Cambridge University brought in a home-built contraption for us to try. It consisted of a metal box containing a single halogen lamp with a copper sheet on top with eppendorf-tube-shaped indentations together with a BBC Micro computer to run it. It was a proto-PCR machine and along with their home-brewed Taq polymerase it worked quite well. This was soon after Saiki et al. 1 had described the full method including the use of a thermostable polymerase and before long manufacturers were offering a dazzling array of PCR machines see 2 including temperature-cycling ovens and water baths neither of which survived the test of time . A few months after we had incorporated the copper contraption into our methodology someone from Archaeology brought along a stingless bee trapped in amber for millions of years. At the time many people saw that PCR could provide a window into the past and there was considerable excitement about its potential even if the number of surviving intact DNA templates was low they could in theory be amplified by PCR. But as with the design of thermo-cycling machines there was a learning curve. Early results from material more than 1 million years old could not be replicated
