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Tuyển tập các báo cáo nghiên cứu về sinh học được đăng trên tạp chí sinh học thế giới đề tài: Estimation of the proportion of genetically unbalanced spermatozoa in the semen of boars carrying | Genet. Sel. Evol. 36 2004 123-137 INRA EDP Sciences 2004 DOI 10.1051 gse 2003055 123 Original article Estimation of the proportion of genetically unbalanced spermatozoa in the semen of boars carrying chromosomal rearrangements using FISH on sperm nuclei Alain PiNTONa Alain Ducosa Martine YERLEb a UMR INRA-ENVT Cytogenetique des populations animales Ecole nationale veterinaire de Toulouse 23 chemin des Capelles 31076 Toulouse Cedex 3 France b Laboratoire de genetique cellulaire Institut national de la recherche agronomique Auzeville BP 27 31326 Castanet-Tolosan Cedex France Received 12 March 2003 accepted 23 May 2003 Abstract - Many chromosomal rearrangements are detected each year in France on young boars candidates for reproduction. The possible use of these animals requires a good knowledge of the potential effect of the rearrangements on the prolificacy of their mates. This effect can be estimated by an accurate determination of the rate of unbalanced spermatozoa in the semen of boars which carry the rearrangements. Indeed these spermatozoa exhibiting normal fertilizing ability are responsible for an early embryonic mortality and then for a decrease of the litter sizes. The spermFISH technique i.e. fluorescent in situ hybridization on decondensed sperm heads has been used on several occasions in Man in this perspective. In livestock species this method was formerly used mainly for semen sexing purposes. We used it for the first time to estimate the rates of imbalance in the semen of four boars carrying chromosomal rearrangements two reciprocal translocations rcp 3 15 q27 q13 and rcp 12 14 q13 q21 as well as two independent cases of trisomy 18 mosaicism. The rates of unbalanced gametes were relatively high for the two reciprocal translocations 47.83 and 24.33 respectively . These values differed from the apparent effects of the rearrangements estimated using a limited number of litters a decrease in prolificacy of 23 estimation obtained using the results of 6 .
