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Tuyển tập các báo cáo nghiên cứu về sinh học được đăng trên tạp chí sinh học thế giới đề tài: Chromosomal rearrangements in cattle and pigs revealed by chromosome microdissection and chromosome painting | 685 Genet Sei. Evol. 35 2003 685-696 INRA EDP Sciences 2003 DOI 10.1051 gse 2003047 Original article Chromosomal rearrangements in cattle and pigs revealed by chromosome microdissection and chromosome painting Alain Pintona Alain Ducosa Martine Yerleb a UMR INRA-ENVT CYtogénétique des populations animales Ecole nationale vétérinaire de Toulouse 23 chemin des Capelles 31076 Toulouse Cedex 3 France b Laboratoire de génétique cellulaire Institut national de la recherche agronomique Auzeville BP 27 31326 Castanet-Tolosan Cedex France Received 12 December 2002 accepted 7 MaY 2003 Abstract - a pericentric inversion of chromosome 4 in a boar as well as a case of 2q 5pC translocation mosaicism in a bull were analysed by chromosome painting using probes generated by conventional microdissection. For the porcine inversion probes specific for p arms and q arms were produced and hybridised simultaneously on metaphases of a heterozygote carrier. In the case of the bovine translocation two whole chromosome probes chromosome 5 and derived chromosome 5 were elaborated and hybridised independently on chromosomal preparations of the bull who was a carrier of the mosaic translocation. The impossibility of differentiating chromosomes 2 and der 2 from other chromosomes of the metaphases did not allow the production of painting probes for these chromosomes. For all experiments the quality of painting was comparable to that usually observed with probes obtained from flow-sorted chromosomes. The results obtained allowed confirmation of the interpretations proposed with G-banding karyotype analyses. In the bovine case however the reciprocity of the translocation could not be proven. The results presented in this paper show the usefulness of the microdissection technique for characterising chromosomal rearrangements in species for which commercial probes are not available. They also confirmed that the main limiting factor of the technique is the quality of the chromosomal preparations which
