Đang chuẩn bị nút TẢI XUỐNG, xin hãy chờ
Tải xuống
Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: SNP high-throughput screening in grapevine using the | BMC Plant Biology BioMed Central Open Access Methodology article SNP high-throughput screening in grapevine using the SNPlex genotyping system Massimo Pindo 1 Silvia Vezzulli1 Giuseppina Coppola1 Dustin A Cartwright2 Andrey Zharkikh2 Riccardo Velasco1 and Michela Troggio1 Address 1IASMA Research Center Via E. Mach 1 - I 38010 - San Michele all Adige Italy and 2Myriad Genetics Inc Wakara Way 320 - UT 84108 - Salt Lake City USA Email Massimo Pindo - massimo.pindo@iasma.it Silvia Vezzulli - silvia.vezzulli@iasma.it Giuseppina Coppola - giuseppina.coppola@iasma.it Dustin A Cartwright - dustin.cartwright@gmail.com Andrey Zharkikh - zharkikh@myriad.com Riccardo Velasco - riccardo.velasco@iasma.it Michela Troggio - michela.troggio@iasma.it Corresponding author Published 28 January 2008 Received 20 September 2007 BMC Plant Biology 2008 8 12 doi 10.1186 1471-2229-8-12 Accepted 28 January 2008 This article is available from http www.biomedcentral.cOm 1471-2229 8 12 2008 Pindo et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract__ Background Until recently only a small number of low- and mid-throughput methods have been used for single nucleotide polymorphism SNP discovery and genotyping in grapevine Vitis vinifera L. . However following completion of the sequence of the highly heterozygous genome of Pinot Noir it has been possible to identify millions of electronic SNPs eSNPs thus providing a valuable source for high-throughput genotyping methods. Results Herein we report the first application of the SNPlex genotyping system in grapevine aiming at the anchoring of an eukaryotic genome. This approach combines robust SNP detection with automated
