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Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Hepatitis C virus NS4B carboxy terminal domain is a membrane binding domain | Virology Journal BioMed Central Research Hepatitis C virus NS4B carboxy terminal domain is a membrane binding domain Jolanda MP Liefhebber1 Bernd W Brandt2 Rene Broer1 Willy JM Spaan1 and Hans C van Leeuwen 1 2 Open Access Address department of Medical Microbiology Center of Infectious Diseases Leiden University Medical Center 2300 RC Leiden the Netherlands and 2Centre for Integrative Bioinformatics IBIVU VU University Amsterdam the Netherlands Email Jolanda MP Liefhebber - J.M.P.Liefhebber@lumc.nl Bernd W Brandt - bwbrandt@few.vu.nl Rene Broer - broer66@yahoo.co.uk Willy JM Spaan - W.J.M.Spaan@lumc.nl Hans C van Leeuwen - H.C.van_Leeuwen@lumc.nl Corresponding author Published 25 May 2009 Received 10 March 2009 Accepted 25 May 2009 Virology Journal 2009 6 62 doi l0.ll86 l 743-422X-6-62 This article is available from http www.virologyj.cOm content 6 1 62 2009 Liefhebber et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Hepatitis C virus HCV induces membrane rearrangements during replication. All HCV proteins are associated to membranes pointing out the importance of membranes for HCV. Non structural protein 4B NS4B has been reported to induce cellular membrane alterations like the membranous web. Four transmembrane segments in the middle of the protein anchor NS4B to membranes. An amphipatic helix at the amino-terminus attaches to membranes as well. The carboxy-terminal domain CTD of NS4B is highly conserved in Hepaciviruses though its function remains unknown. Results A cytosolic localization is predicted for the NS4B-CTD. However using membrane floatation assays and immunofluorescence we now show targeting of the NS4B-CTD to membranes. Furthermore a profile-profile search with an HCV NS4B-CTD .
