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Glycolysis in the human parasiteEntamoeba histolyticais characterized by the absence of cooperative modulation and the prevalence of pyrophosphate-dependent (over ATP-dependent) enzymes. To determine the flux-control dis-tribution of glycolysis and understand its underlying control mechanisms, a kinetic model of the pathway was constructed by using the softwaregepasi. | ễFEBS Journal Kinetic modeling can describe in vivo glycolysis in Entamoeba histolytica Emma Saavedra1 Alvaro Marin-Hernandez1 Rusely Encalada1 Alfonso Olivos2 Guillermo Mendoza-Hernandez3 and Rafael Moreno-Sanchez1 1 Departamento de Bioquimica Institute Nacionalde Cardiologia Mexico DF Mexico 2 Departamento de Medicina Experimental Facultad de Medicina Universidad NacionalAutonoma de Mexico Mexico DF Mexico 3 Departamento de Bioquimica Facultad de Medicina Universidad NacionalAutonoma de Mexico Mexico DF Mexico Keywords ATPases drug targeting hexokinase phosphoglycerate mutase Correspondence E. Saavedra Departamento de Bioquimica Instituto Nacionalde Cardiologia Juan Badiano no. 1 Col. Seccion XVI CP 14080 Tlalpan Mexico DF Mexico Fax 5255 5573 0926 Tel 5255 5573 2911 ext. 1422 E-mail emma_saavedra2002@yahoo.com Note The mathematicalmodeldescribed here has been submitted to the Online Cellular Systems Modelling Database and can be accessed at http jjj.biochem.sun.ac.za database saavedra index.htmlfree of charge Received 7 November 2006 revised 13 July 2007 accepted 27 July 2007 doi 10.1111 j.1742-4658.2007.06012.x Glycolysis in the human parasite Entamoeba histolytica is characterized by the absence of cooperative modulation and the prevalence of pyrophosphatedependent over ATP-dependent enzymes. To determine the flux-control distribution of glycolysis and understand its underlying control mechanisms a kinetic model of the pathway was constructed by using the software GEPASI. The model was based on the kinetic parameters determined in the purified recombinant enzymes and the enzyme activities and steady-state fluxes and metabolite concentrations determined in amoebal trophozoites. The model predicted with a high degree of accuracy the flux and metabolite concentrations found in trophozoites but only when the pyrophosphate concentration was held constant at variable pyrophosphate the model was not able to completely account for the ATP production consumption .
