Đang chuẩn bị nút TẢI XUỐNG, xin hãy chờ
Tải xuống
Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Expression and characterization of the UL31 protein from duck enteritis virus | Virology Journal BioMed Central Open Access Expression and characterization of the UL31 protein from duck enteritis virus Wei Xie1 Anchun Cheng 11 2 Mingshu Wang11 2 Hua Chang2 Dekang Zhu1 2 Qihui Luo2 Renyong Jia2 and Xiaoyue Chen2 Address 1Avian Diseases Research Center College of Veterinary Medicine of Sichuan Agricultural University Ya an Sichuan 625014 PR China and 2Key Laboratory of Animal Diseases and Human Health of Sichuan Province Ya an Sichuan 625014 PR China Email Wei Xie - xiew8112@163.com Anchun Cheng - chenganchun@hotmail.com Mingshu Wang - mshwang@163.com Hua Chang-changhuaxx@126.com Dekang Zhu - zdk24@163.com Qihui Luo - luoqihui80@163.com RenyongJia-cqrcjry@yahoo.com.cn Xiaoyue Chen - chenxy@163.com Corresponding author fEqual contributors Published 10 February 2009 Received 28 December 2008 Accepted 10 February 2009 Virology journal 2009 6 19 doi l0.ll86 l743-422X-6-l9 This article is available from http www.virologyj.cOm content 6 l l9 2009 Xie et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Previous studies indicate that the UL3l protein and its homology play similar roles in nuclear egress of all herpesviruses. However there is no report on the UL3l gene product of DEV. In this study we expressed and presented the basic properties of the DEV UL3l product. Results The entire ORF of the UL3l was cloned into pET 32a prokaryotic expression vector. Escherichia coli BL2l DE3 competent cells were transformed with the construct followed by the induction of protein expression by the addition of IPTG. Band corresponding to the predicted sizes 55 kDa was produced on the SDS-PAGE. Over expressed 6xHis-UL3l fusion protein was purified by nickel affinity chromatography. The DEV UL3 l gene .
