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Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Expression of Ebolavirus glycoprotein on the target cells enhances viral entry | Virology Journal BioMed Central Research Expression of Ebolavirus glycoprotein on the target cells enhances viral entry Balaji Manicassamy1 2 and Lijun Rong 1 Address Department of Microbiology and Immunology College of Medicine University of Illinois at Chicago Chicago Illinois USA and 2Department of Microbiology Mount Sinai School of Medicine 1 Gustave L Levy Place Box 1124 New York New York USA Email Balaji Manicassamy-balaji.manicassamy@mssm.edu Lijun Rong - lijun@uic.edu Corresponding author Open Access Published 8 June 2009 Received I April 2009 Accepted 8 June 2009 Virology journal 2009 6 75 doi 10.1 186 1743-422X-6-75 This article is available from http www.virologyj.cOm content 6 1 75 2009 Manicassamy and Rong licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract_ Background Entry of Ebolavirus to the target cells is mediated by the viral glycoprotein GP. The native GP exists as a homotrimer on the virions and contains two subunits a surface subunit GP1 that is involved in receptor binding and a transmembrane subunit GP2 that mediates the virushost membrane fusion. Previously we showed that over-expression of GP on the target cells blocks GP-mediated viral entry which is mostly likely due to receptor interference by GP1. Results In this study using a tetracycline inducible system we report that low levels of GP expression on the target cells instead of interfering specifically enhance GP mediated viral entry. Detailed mapping analysis strongly suggests that the fusion subunit GP2 is primarily responsible for this novel phenomenon here referred to as trans enhancement. Conclusion Our data suggests that GP2 mediated trans enhancement
