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Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Conserved retinoblastoma protein-binding motif in human cytomegalovirus UL97 kinase minimally impacts viral replication but affects susceptibility to maribavir | Virology Journal BioMed Central Open Access Short report Conserved retinoblastoma protein-binding motif in human cytomegalovirus UL97 kinase minimally impacts viral replication but affects susceptibility to maribavir Rachel B Gill 1 3 Samuel L Frederick11 Caroll B Hartline1 Sunwen Chou2 and Mark N Prichard 1 Address Department of Pediatrics University of Alabama School of Medicine Birmingham AL USA 2Medical and Research Services VA Medical Center and Oregon Health and Science University Portland OR USA and 3Department of Cell Biology University of Alabama School of Medicine Birmingham AL USA Email Rachel B Gill - rbgill@uab.edu Samuel L Frederick - sfrederick@peds.uab.edu Caroll B Hartline - chartline@peds.uab.edu Sunwen Chou - chous@ohsu.edu MarkN Prichard - mprichard@peds.uab.edu Corresponding author fEqual contributors Published 21 January 2009 Received 29 December 2008 Accepted 21 January 2009 Virology Journal 2009 6 9 doi l0.ll86 l743-422X-6-9 This article is available from http www.virologyj.cOm content 6 1 9 2009 Gill et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract The UL97 kinase has been shown to phosphorylate and inactivate the retinoblastoma protein Rb and has three consensus Rb-binding motifs that might contribute to this activity. Recombinant viruses containing mutations in the Rb-binding motifs generally replicated well in human foreskin fibroblasts with only a slight delay in replication kinetics. Their susceptibility to the specific UL97 kinase inhibitor maribavir was also examined. Mutation of the amino terminal motif which is involved in the inactivation of Rb also renders the virus hypersensitive to the drug and suggests that the motif may play a role in its mechanism of action. Findings .
