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Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: Large-scale identification of human genes implicated in epidermal barrier function. | Open Access Large-scale identification of human genes implicated in epidermal barrier function Eve Toulza Nicolas R Mattiuzzo Marie-Florence Galliano Nathalie Jonca Carole Dossat Daniel Jacob Antoine de Daruvar Patrick Wincker Guy Serre and Marina Guerrin Addresses UMR 5165 Epidermis Differentiation and Rheumatoid Autoimmunity CNRS - Toulouse III University IFR 30 INSERM - CNRS - Toulouse III University - CHU allées Jules Guesde 31073 Toulouse France. Genoscope and CNRS UMR 8030 rue Gaston Crémieux 91057 Evry France. Centre de Bioinformatique Bordeaux Université V. Segalen Bordeaux 2 rue Léo Saignat 33076 Bordeaux Cedex France. Correspondence Marina Guerrin. Email mweber@udear.cnrs.fr Published II June 2007 Genome Biology 2007 8 RI07 doi I0.II86 gb-2007-8-6-rI 07 The electronic version of this article is the complete one and can be found online at http genomebiology.com 2007 8 6 RI07 Received I March 2007 Revised 24 May 2007 Accepted II June 2007 2007 Toulza et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background During epidermal differentiation keratinocytes progressing through the suprabasal layers undergo complex and tightly regulated biochemical modifications leading to cornification and desquamation. The last living cells the granular keratinocytes GKs produce almost all of the proteins and lipids required for the protective barrier function before their programmed cell death gives rise to corneocytes. We present here the first analysis of the transcriptome of human GKs purified from healthy epidermis by an original approach. Results Using the ORESTES method 22 585 expressed sequence tags ESTs were produced that matched 3 387 genes. Despite normalization provided by this method mean 4.6 ORESTES per .
