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Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: RAD marker microarrays enable rapid mapping of zebrafish mutations. | Open Access RAD marker microarrays enable rapid mapping of zebrafish mutations Michael R Miller Tressa S Atwood B Frank Eames Johann K Eberhart Yi-Lin Yan John H Postlethwait and Eric A Johnson Addresses institute of Molecular Biology University of Oregon 1370 Franklin Blvd. Eugene Oregon 97403 USA. Institute of Neuroscience University of Oregon 1370 Franklin Blvd. Eugene Oregon 97403 USA. FloraGenex Inc. 1370 Franklin Blvd. Eugene Oregon 97403 USA. Correspondence Eric A Johnson. Email eric-johnson@molbio.uoregon.edu Published 6 June 2007 Genome Biology 2007 8 R105 doi l0.ll86 gb-2007-8-6-rl 05 The electronic version of this article is the complete one and can be found online at http genomebiology.com 2007 8 6 R105 Received 12 January 2007 Revised 2 April 2007 Accepted 6 June 2007 2007 Miller et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract We constructed a restriction site associated DNA RAD marker microarray to facilitate rapid genetic mapping of zebrafish mutations. Using these microarrays with a bulk segregant approach we localized previously unmapped mutations to genomic regions just a few centiMorgans in length. Furthermore we developed an approach to assay individual RAD markers in pooled populations and refined one region. The RAD approach is highly effective for genetic mapping in zebrafish and is an attractive option for mapping in other organisms. Background Zebrafish Danio rerio has become an important model system for the study of vertebrate development and human disease for the assignment of function to genes otherwise known only by sequence and for the identification of novel functions for genes with previously described functions 1-5 . The success of zebrafish investigation has in large part .
