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Inhibition of Tomato Yellow Leaf Curl Virus (TYLCV) using whey proteins | Abdelbacki et al. Virology Journal 2010 7 26 http www.virologyj.eom content 7 1 26 VIROLOGY JOURNAL RESEARCH Open Access Inhibition of Tomato Yellow Leaf Curl Virus TYLCV using whey proteins Ashraf M Abdelbacki1 Soad H Taha2 Mahmoud Z Sitohy3 Abdelgawad I Abou Dawood2 Mahmoud M Abd-El Hamid2 Adel A Rezk4 Abstract The antiviral activity of native and esterified whey proteins fractions a-lactalbumin p-lactoglobulin and lactoferrin was studied to inhibit tomato yellow leaf curl virus TYLCV on infected tomato plants. Whey proteins fractions and their esterified derivatives were sprayed into TYLCV-infected plants. Samples were collected from infected leaves before treatment 7 and 15 days after treatment for DNA and molecular hybridization analysis. The most evident inhibition of virus replication was observed after 7 and 15 days using a-lactoferrin and a-lactalbumin respectively. Native and esterified lactoferrin showed complete inhibition after 7 days. On the other hand native p-lactoglobulin showed inhibition after 7 and 15 days whereas esterified p-lactoglobulin was comparatively more effective after 7 days. The relative amount of viral DNA was less affected by the esterified a-lactalbumin whereas native a-lactalbu-min inhibited virus replication completely after 15 days. These results indicate that native or modified whey proteins fractions can be used for controlling the TYLCV-infected plants. Introduction Tomato yellow leaf curl virus TYLCV is one of the major and serious diseases of tomato which causes considerable amount of yield loss in Egypt 1-3 . One hundred twenty five million tons of tomatoes were produced in the world in 2007. China the largest producer accounted for about one quarter of the global output followed by the United States Turkey India and Egypt. http www.fas.usda.gov htp 2009 20Tomato 20Article.pdf. Losses from plant diseases can have a significant economic impact causing a reduction in income for crop producers distributors and higher prices
