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Detection of dengue virus in saliva and urine by real time RT-PCR | Poloni et al. Virology Journal 2010 7 22 http www.virologyj.eom content 7 1 22 VIROLOGY JOURNAL SHORT REPORT Open Access Detection of dengue virus in saliva and urine by real time RT-PCR Telma R Poloni 1 Anibal S Oliveira1 Helda L Alfonso1 Larissa R Galvão1 Alberto A Amarilla2 Dimair F Poloni3 Luiz T Figueiredo2 Victor H Aquino1 Abstract Early diagnosis of dengue virus DENV infection is important for patient management and control of dengue outbreaks. The objective of this study was to analyze the usefulness of urine and saliva samples for early diagnosis of DENV infection by real time RT-PCR. Two febrile patients who have been attended at the General Hospital of the School of Medicine of Ribeirao Preto Sao Paulo University were included in the study. Serum urine and saliva samples collected from both patients were subjected to real time RT-PCR for DENV detection and quantification. Dengue RNA was detected in serum urine and saliva samples of both patients. Patient 1 was infected with DENV-2 and patient 2 with DENV-3. Data presented in this study suggest that urine and saliva could be used as alternative samples for early diagnosis of dengue virus infection when blood samples are difficult to obtain e.g. in newborns and patients with hemorrhagic syndromes. Finding Infection with any of the four dengue virus serotypes can lead to a broad clinical spectrum ranging from sub-clinical infection or an influenza-like disease known as dengue fever DF to a severe sometimes fatal disease characterized by hemorrhage and plasma leakage that can lead to shock known as dengue hemorrhagic fever dengue shock syndrome DHF DSS 1 2 . Laboratory diagnosis of DENV infection is based on virus isolation detection of virus antigen or RNA and detection of DENV specific antibodies in serum or plasma 3 . Realtime reverse transcription-polymerase chain reaction RT-PCR has been developed to detect DENV RNA in human samples 4-7 . This technique is an important tool for the rapid diagnosis of .
