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Cellulose and wheat straw degradation byRuminococcus albuswas moni-tored using NMR spectroscopy. In situ solid-state 13 C-cross-polarization magic angle spinning NMR was used to monitor the modification of the composition and structure of cellulose and 13 C-enriched wheat straw during the growth of the bacterium on these substrates. | ỊFEBS Journal NMR study of cellulose and wheat straw degradation by Ruminococcus albus 20 Maria Matulova1 2 Regis Nouaille2 3 Peter Capek1 Michel Pean4 5 6 Anne-Marie Delort2 and Evelyne Forano3 1 Institute of Chemistry Slovak Academy of Sciences Centre for Glycomics Bratislava Slovak Republic 2 Laboratoire de Synthese et Etude de Systemes a Interet Biologique UMR 6504 Universite Blaise Pascal CNRS Aubiere France 3 INRA UR 454 Unite de Microbiologie Centre de Recherches de Clermont-Ferrand-Theix Saint-Genes-Champanelle France 4 CEA DSV IBEB Groupe Recherches Appliques Phytotechnologie Saint-Paul-lez-Durance France 5 CNRS UMR Biologie Vegetale Microbiolgie Environnementale Saint-Paul-lez-Durance France 6 Aix-Marseille Universite Saint-Paul-lez-Durance France Keywords cellulose NMR rumen Ruminococcus albus wheat straw Correspondence E. Forano INRA Unite de Microbiologie Centre de Recherches de Clermont-Ferrand-Theix 63122 Saint-Genes-Champanelle France Fax 33 473 62 45 81 Tel 33 473 62 42 48 E-mail forano@clermont.inra.fr Received 25 February 2008 revised 7 May 2008 accepted 7 May 2008 doi 10.1111 j.1742-4658.2008.06497.x Cellulose and wheat straw degradation by Ruminococcus albus was monitored using NMR spectroscopy. In situ solid-state 13C-cross-polarization magic angle spinning NMR was used to monitor the modification of the composition and structure of cellulose and 13C-enriched wheat straw during the growth of the bacterium on these substrates. In cellulose amorphous regions were not preferentially degraded relative to crystalline areas by R. albus. Cellulose and hemicelluloses were also degraded at the same rate in wheat straw. Liquid state two-dimensional NMR experiments were used to analyse in detail the sugars released in the culture medium and the integration of NMR signals enabled their quantification at various times of culture. The results showed glucose and cellodextrin accumulation in the medium of cellulose cultures the cellodextrins were mainly .
