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Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Insecticidal activity of two proteases against Spodoptera frugiperda larvae infected with recombinant baculoviruses Aline Welzel Gramkow, Simone Perecmanis, Raul Lim | Gramkow et al. Virology Journal 2010 7 143 http www.virologyj.eom content 7 1 143 VIROLOGY JOURNAL RESEARCH Open Access Insecticidal activity of two proteases against Spodoptera frugiperda larvae infected with recombinant baculoviruses Aline Welzel Gramkow Simone Perecmanis Raul Lima Barbosa Sousa Eliane Ferreira Noronha Carlos Roberto Felix Tatsuya Nagata and Bergmann Morais Ribeiro Abstract Background Baculovirus comprise the largest group of insect viruses most studied worldwide mainly because they efficiently kill agricutural insect pests. In this study two recombinant baculoviruses containing the ScathL gene from Sarcophaga peregrina vSynScathL and the Keratinase gene from the fungus Aspergillus fumigatus vSynKerat were constructed. and their insecticidal properties analysed against Spodoptera frugiperda larvae. Results Bioassays of third-instar and neonate S. frugiperda larvae with vSynScathL and vSynKerat showed a decrease in the time needed to kill the infected insects when compared to the wild type virus. We have also shown that both recombinants were able to increase phenoloxidase activity in the hemolymph of S. frugiperda larvae. The expression of proteases in infected larvae resulted in destruction of internal tissues late in infection which could be the reason for the increased viral speed of kill. Conclusions Baculoviruses and their recombinant forms constitute viable alternatives to chemical insecticides. Recombinant baculoviruses containing protease genes can be added to the list of engineered baculoviruses with great potential to be used in integrated pest management programs. Background Baculovirus comprise the largest group of insect viruses most studied worldwide mainly because they efficiently kill agricultural insect pests. They are specific to one or a few related insect species 1 and have infectious particles protected in protein crystals which allows the formulation of biopesticides with easy application technology. Their use as .
