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Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Design, assembly, and validation of a nose-only inhalation exposure system for studies of aerosolized viable influenza H5N1 virus in ferrets | Tuttle et al. Virology Journal 2010 7 135 http www.virologyj.eom content 7 1 135 VIROLOGY JOURNAL METHODOLOGY Open Access Designl assembly and validation of a nose-only inhalation exposure system for studies of aerosolized viable influenza H5N1 virus in ferrets Richard S Tuttle1 William A Sosna2 Deirdre E Daniels2 Sara B Hamilton2 and John A Lednicky 2 Abstract Background The routes by which humans acquire influenza H5N1 infections have not been fully elucidated. Based on the known biology of influenza viruses four modes of transmission are most likely in humans aerosol transmission ingestion of undercooked contaminated infected poultry transmission by large droplets and self-inoculation of the nasal mucosa by contaminated hands. In preparation of a study to resolve whether H5N1 viruses are transmissible by aerosol in an animal model that is a surrogate for humans an inhalation exposure system for studies of aerosolized H5N1 viruses in ferrets was designed assembled and validated. Particular attention was paid towards system safety efficacy of dissemination the viability of aerosolized virus and sampling methodology. Results An aerosol generation and delivery system referred to as a Nose-Only Bioaerosol Exposure System NBIES was assembled and function tested. The NBIES passed all safety tests met expected engineering parameters required relatively small quantities of material to obtain the desired aerosol concentrations of influenza virus and delivered doses with high-efficacy. Ferrets withstood a mock exposure trial without signs of stress. Conclusions The NBIES delivers doses of aerosolized influenza viruses with high efficacy and uses less starting material than other similar designs. Influenza H5N1 and H3N2 viruses remain stable under the conditions used for aerosol generation and sample collection. The NBIES is qualified for studies of aerosolized H5N1 virus. Background Human infections caused by highly pathogenic avian influenza H5N1 viruses H5N1 that arose .
