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The positive cofactor 4 (PC4) protein has an important role in transcrip-tional activation, which has been proposed to be mediated by transcription factor IIA (TFIIA) and TATA-binding protein-associated factors. To test this hypothesis, we cloned the Schizosaccharomyces pombe PC4gene and analysed the role of the PC4 protein in the stimulation of basal tran-scription driven by TATA-containing and TATA-less promoters. | ỊFEBS Journal Schizosaccharomyces pombe positive cofactor 4 stimulates basal transcription from TATA-containing and TATA-less promoters through Mediator and transcription factor IIA Juan Contreras-Levicoy Fabiola Urbina and Edio Maldonado Programa de Biologia Celular y Molecular Facultad de Medicina Institute de Ciencias Biomedicas Universidad de Chile Santiago Chile Keywords PC4 promoter RNAPII stimulation transcription Correspondence E. Maldonado Programa de Biologia Celular y Molecular Facultad de Medicina Instituto de Ciencias Biomedicas Universidad de Chile Casilla 70086 Santiago 7 Chile Fax 56 2 735 5580 Tel 56 2 978 6207 E-mail emaldona@med.uchile.cl These authors contributed equally to this work Received 21 January 2008 revised 6 March 2008 accepted 31 March 2008 The positive cofactor 4 PC4 protein has an important role in transcriptional activation which has been proposed to be mediated by transcription factor IIA TFIIA and TATA-binding protein-associated factors. To test this hypothesis we cloned the Schizosaccharomyces pombe PC4 gene and analysed the role of the PC4 protein in the stimulation of basal transcription driven by TATA-containing and TATA-less promoters. Sc. pombe PC4 was able to stimulate basal transcription from several TATA-contain-ing promoters and from the Initiator sequences of the highly transcribed Sc. pombe nmtl gene. Moreover it was demonstrated that Sc. pombe PC4 stimulates formation of the transcription preinitiation complex. Activation of transcription by PC4 was dependent on the Mediator complex and TFIIA but was independent of TATA-binding protein-associated factor. PC4 binds to double-stranded and single-stranded DNA and interacts with TATA-binding protein TFIIB TFIIA Mediator TFIIH and the transcriptional activator protein VP16. doi 10.1111 j.1742-4658.2008.06429.x The transcription of protein-coding genes is carried out by RNA polymerase II RNAPII and six general transcription factors GTFs called TFIIA TFIIB TFIID TFIIE TFIIF
