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Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: AtKinesin-13A is located on Golgi-associated vesicle and involved in vesicle formation/budding in Arabidopsis root-cap peripheral cells | BMC Plant Biology BioMed Central Research article AtKinesin-13A is located on Golgi-associated vesicle and involved in vesicle formation budding in Arabidopsis root-cap peripheral cells Liqin Wei11 2 Wei Zhang11 Zhaohui Liu1 and Yan Li 1 Open Access Address 1State Key Laboratory of Plant Physiology and Biochemistry College of Biological Sciences China Agricultural University Beijing 100193 PR China and 2Research Center of Molecular and Developmental Biology Key Laboratory of Photosynthesis and Environmental Molecular Physiology Institute of Botany Chinese Academy of Sciences Beijing 100093 PR China Email Liqin Wei - weiliqin@ibcas.ac.cn Wei Zhang - wei_zhang6423@ 126.com Zhaohui Liu - zhaoh@cau.edu.cn Yan Li - liyan@cau.edu.cn Corresponding author fEqual contributors Published 25 November 2009 Received 19 March 2009 BMC Plant Biology 2009 9 138 doi 10.1186 1471 -2229-9-138 Accepted 25 November 2009 This article is available from http www.biomedcentral.cOm 1471-2229 9 138 2009 Wei et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background AtKinesin-13A is an internal-motor kinesin from Arabidopsis Arabidopsis thaliana . Previous immunofluorescent results showed that AtKinesin-13A localized to Golgi stacks in plant cells. However its precise localization and biological function in Golgi apparatus is unclear. Results In this paper immunofluorescent labeling and confocal microscopic observation revealed that AtKinesin-13A was co-localized with Golgi stacks in Arabidopsis root tip cells. Immunoelectron microscopic observations indicated that AtKinesin-13A is primarily localized on Golgi-associated vesicles in Arabidopsis root-cap cells. By T-DNA insertion the inactivation of the AtKinesin-l3A gene NM-112536 .
