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Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: A novel totivirus and piscine reovirus (PRV) in Atlantic salmon (Salmo salar) with cardiomyopathy syndrome (CMS) | Lovoll et al. Virology Journal 2010 7 309 http www.virologyj.eom content 7 1 309 VIROLOGY JOURNAL RESEARCH Open Access A novel totivirus and piscine reovirus PRV in Atlantic salmon Salmo salar with cardiomyopathy syndrome CMS 1 2 I- f 1 1 3 1 Marie Lovoll Jannicke Wiik-Nielsen S0ren Grove Christer R Wiik-Nielsen Anja B Kristoffersen Randi Faller Tn m o Dr it r iCi4 looni I II m 5 F 212 r 2 c DarH m2 I II I5 A Iav2 nHar I MoHorhi ri f 6 í l 2i 1 k ií2 A h lax arcmn7 liygve Poppe Joonil Jung Ciiandia S Pedamallu Alexander J Nedeiuiagi Matthew Meyeison Espen Rimstad8 Torstein Tengs9 Abstract Background Cardiomyopathy syndrome CMS is a severe disease affecting large farmed Atlantic salmon. Mortality often appears without prior clinical signs typically shortly prior to slaughter. We recently reported the finding and the complete genomic sequence of a novel piscine reovirus PRV which is associated with another cardiac disease in Atlantic salmon heart and skeletal muscle inflammation HSMI . In the present work we have studied whether PRV or other infectious agents may be involved in the etiology of CMS. Results Using high throughput sequencing on heart samples from natural outbreaks of CMS and from fish experimentally challenged with material from fish diagnosed with CMS a high number of sequence reads identical to the rRV genome were identified. In addition a sequence contig from a novel totivirus could also be constructed. Using RT-qrCR levels of rRV in tissue samples were quantified and the totivirus was detected in all samples tested from CMS fish but not in controls. n situ hybridization supported this pattern indicating a possible association between CMS and the novel piscine totivirus. Conclusions Although causality for CMS in Atlantic salmon could not be proven for either of the two viruses our results are compatible with a hypothesis where in the experimental challenge studied rRV behaves as an opportunist whereas the totivirus might be more directly linked with
