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Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài: Severe endothelial injury and subsequent repair in patients after successful . | Fink et al. Critical Care 2010 14 R104 http ccforum.eom content 14 3 R104 c CRITICAL CARE RESEARCH Open Access Severe endothelial injury and subsequent repair in patients after successful cardiopulmonary resuscitation Katrin Fink 21 Meike Schwarz21 Linda Feldbrugge 1 Julia N Sunkomat1 Tilmann Schwab1 Natascha Bourgeois1 Manfred Olschewski2 Constantin von zur Muhlen1 Christoph Bode1 and Hans-Jorg Busch1 Abstract Introduction Ischemia and reperfusion after cardiopulmonary resuscitation CPR induce endothelial activation and systemic inflammatory response resulting in post-resuscitation disease. In this study we analyzed direct markers of endothelial injury circulating endothelial cells CECs and endothelial microparticles EMPs and endothelial progenitor cells EPCs as a marker of endothelial repair in patients after CPR. Methods First we investigated endothelial injury in 40 patients after CPR 30 controls with stable coronary artery disease CAD and 9 healthy subjects who were included to measure CECs and EMPs. In a subsequent study endothelial repair was assessed by EPC measurement in 15 CPR 9 CAD and 5 healthy subjects. Blood samples were drawn immediately and 24 hours after ROSC and analyzed by flow cytometry. For all statistical analyses P 0.05 was considered significant. Results There was a massive rise in CEC count in resuscitated patients compared to CAD 4 494.1 1 246 versus 312.7 41 cells mL P 0.001 and healthy patients 47.5 3.7 cells mL P 0.0005 . Patients after prolonged CPR 30 min showed elevated CECs compared to those resuscitated for 30 min 6 216.6 2 057 versus 2 340.9 703.5 cells mL P 0.13 ns . There was a significant positive correlation of CEC count with duration of CPR R2 0.84 P 0.01 . EMPs were higher immediately after CPR compared to controls 31.2 5.8 versus 19.7 2.4 events pL P 0.12 CAD versus 15.0 5.2 events pL P 0.07 healthy but did not reach significance until 24 hours after CPR 69.1 12.4 versus 22.0 3.0 events pL P 0.005 CAD versus 15.4 4.4 events