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Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài:A network model for biofilm development in Escherichia coli K-12 | Shalá et al. Theoretical Biology and Medical Modelling 2011 8 34 http www.tbiomed.eom content 8 1 34 THEORETICAL BIOLOGY AND MEDICAL MODELLING RESEARCH Open Access A network model for biofilm development in Escherichia coli K-12 Andrew A Shalá1 Silvia Restrepo2 and Andrés F Gonzalez Barrios1 Correspondence andgonza@uniandes.edu.co 1Grupo de Diseno de Productos y Procesos GDPP Departamento de Ingeniería Química Universidad de los Andes. Carrera 1E No. 19a14 Bogota Colombia Full list of author information is available at the end of the article 2 BioMed Central Abstract Background In nature bacteria often exist as biofilms. Biofilms are communities of microorganisms attached to a surface. It is clear that biofilm-grown cells harbor properties remarkably distinct from planktonic cells. Biofilms frequently complicate treatments of infections by protecting bacteria from the immune system decreasing antibiotic efficacy and dispersing planktonic cells to distant body sites. In this work we employed enhanced Boolean algebra to model biofilm formation. Results The network obtained describes biofilm formation successfully assuming -in accordance with the literature - that when the negative regulators RscCD and EnvZ OmpR are off the positive regulator FlhDC is on. The network was modeled under three different conditions through time with satisfactory outcomes. Each cluster was constructed using the K-means medians Clustering Support algorithm on the basis of published Affymetrix microarray gene expression data from biofilmforming bacteria and the planktonic state over four time points for Escherichia coli IK-12. Conclusions The different phenotypes obtained demonstrate that the network model of biofilm formation can simulate the formation or repression of biofilm efficiently in E. coli K-12. Background In natural medical or engineering environments bacteria often exist as sessile communities called biofilms 1 which are exquisite structures caused by a genetically programmed .
