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Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài: "Identity of zinc finger nucleases with specificity to herpes simplex virus type II genomic DNA: novel HSV-2 vaccine/therapy precursors | Wayengera Theoretical Biology and Medical Modelling 2011 8 23 http www.tbiomed.eom content 8 1 23 THEORETICAL BIOLOGY AND MEDICAL MODELLING RESEARCH Open Access Identity of zinc finger nucleases with specificity to herpes simplex virus type II genomic DNA novel HSV-2 vaccine therapy precursors Misaki Wayengera Correspondence wmisaki@yahoo. com Unit of Genetics Genomics Theoretical Biology Dept of Pathology School of Biomedical Science College of Health Sciences Makerere University. P o Box 7072 Kampala Uganda 2 BioMed Central Abstract Background Herpes simplex type II HSV-2 is a member of the family herpesviridae. Human infection with this double stranded linear DNA virus causes genital ulcerative disease and existing treatment options only serve to resolve the symptomatology ulcers associated with active HSV-2 infection but do not eliminate latent virus. As a result infection with HSV-2 follows a life-long relapsing active versus latent course. On the basis of a primitive bacterium anti-phage DNA defense the restriction modification R-M system we previously identified the Escherichia coli restriction enzyme REase EcoRII as a novel peptide to excise or irreversibly disrupt latent HSV-2 DNA from infected cells. However sequences of the site specificity palindrome of EcoRII 5 -CCWGG-3 W A or T are equally present within the human genome and are a potential source of host-genome toxicity. This feature has limited previous HSV-2 EcoRII based therapeutic models to microbicides only and highlights the need to engineer artificial REases zinc finger nucleases-ZFNs with specificity to HSV-2 genomic-DNA only. Herein the therapeutic-potential of zinc finger arrays ZFAs and ZFNs is identified and modeled with unique specificity to the HSV-2 genome. Methods and results Using the whole genome of HSV-2 strain HG52 Dolan A et al. and with the ZFN-consortium s CoDA-ZiFiT software pre-set at default more than 28 000 ZFAs with specificity to HSV-2 DNA were identified. Using .
