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Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: Assessment of allergen cross-reactivity. | Clinical and Molecular Allergy BioMed Central Commentary Assessment of allergen cross-reactivity Rob C Aalberse 1 2 Open Access Address 1Sanquin Research and Karl Landsteiner Laboratory Academic Medical Center University of Amsterdam Amsterdam The Netherlands and 2Department of Immunopathology Sanquin Research Plesmanlaan 125 NL 1066 CX Amsterdam The Netherlands Email Rob C Aalberse - r.aalberse@sanquin.nl Corresponding author Published 9 February 2007 Received 23 January 2007 Clinical and Molecular Allergy 2007 5 2 doi l0.ll 86 1476-7961-5-2 Accepted 9 February 2007 This article is available from http www.clinicalmolecularallergy.cOm content 5 1 2 2007 Aalberse licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract The prediction of allergen cross-reactivity is currently largely based on linear sequence data but will soon include 3D information on homology among surface exposed residues. To evaluate procedures for these predictions we need ways to quantitatively assess actual cross-reactivity between two allergens. Three parameters are mentioned 1 the fraction of the epitopes that is cross-reactive 2 the fraction of IgE that is cross- reactive 3 the relative affinity of the interaction between IgE and the two allergens. This editorial briefly compares direct binding protocols with the often more appropriate reciprocal inhibition protocols. The latter type of protocol provides information on symmetric versus asymmetric cross-reactivity and thus on the distinction between complete sensitising allergens versus incomplete cross-reacting allergens. The need to define the affinity threshold of the assay and a caveat on the use of serum pools are also discussed. In a paper recently published in this Journal the question was .
