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Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: Assessment and histological analysis of the IPRL technique for sequential in situ liver biopsy. | Rowe et al. Comparative Hepatology 2011 10 7 http www.comparative-hepatology.eom content 10 1 7 COMPARATIVE HEPATOLOGY RESEARCH Open Access Assessment and histological analysis of the IPRL technique for sequential in situ liver biopsy Anthony Rowe 1 Lillian Zhang1 Azmena Hussain1 Filip Braet2 and Iqbal Ramzan1 Abstract Background The isolated perfused rat liver IPRL is a technique used in a wide range of liver studies. Typically livers are assessed at treatment end point. Techniques have been described to biopsy liver in the live rat and post-hepatectomy. Results This paper describes a technique for obtaining two full and one partial lobe biopsies from the liver in situ during an IPRL experiment. Our approach of retaining the liver in situ assists in minimising liver capsule damage and consequent leakage of perfusate maintains the normal anatomical position of the liver during perfusion and helps to keep the liver warm and moist. Histological results from sequential lobe biopsies in control perfusions show that cytoplasmic vacuolation of hepatocytes is a sign of liver deterioration and when it occurs it commences as a diffuse pattern which tends to develop a circumscribed centrilobular pattern as perfusion progresses. Conclusions Liver lobe biopsies obtained using this method can be used to study temporal effects of drug treatments and are suitable for light and electron microscopy and biochemical analyses. Background The isolated perfused rat liver IPRL is a well characterised model which is commonly used to study the biology and pathobiology of the liver in various experimental settings 1-3 . IPRL has a wide range of applications including ischemia-reperfusion 4 biochemistry 5 pharmacology 6 and immunology 7 . Previous and ongoing studies in our laboratory have used this model to examine the hepatotoxicity of kava 8 . Liver lobe biopsies during IPRL enable temporal profiles of treatments to be observed in each liver. Lobe biopsy techniques have been described .
