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Tải xuống
In the study "Optimization of Agrobacterium-mediated transformation and regeneration for CRISPR/Cas9 genome editing of commercial tomato cultivars", we have optimized an Agrobacterium-mediated gene transfer and regeneration system for CRISPR/Cas9 genome editing in two commercial tomato cultivars for the first time. The effect of explant type, genotype, pre-transformation time, Agrobacterium concentration, infection time, and different co-culture periods of bacteria were evaluated to optimize the regeneration and transformation parameters. |
