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Inhibitory effect of oil and its preparations were studied using disc-diffusion and Minimum Inhibitory Concentration (MIC) tests, and using corn oil and Streptomycin as controls. | Annals of Agricultural Science 2015 60 2 335 340 H O S T E D BY Faculty of Agriculture Ain Shams University Annals of Agricultural Science www.elsevier.com locate aoas Anti-microbial cytotoxicity and necrotic ripostes of Pimpinella anisum essential oil Mohammed A.T. Abdel-Reheem a Mona M. Oraby b a Biochemistry Dept. Faculty of Agric. Ain Shams Univ. Cairo Egypt b Agric. Microbiology Dept. Faculty of Agric. Ain Shams Univ. Cairo Egypt Received 4 October 2015 accepted 13 October 2015 Available online 27 November 2015 KEYWORDS Abstract Pimpinella anisum essential oil EO residuals and its biotic possessions were deliberated. Anise essential oil EO was identified using UPLC MS MS analysis. Inhibitory effect of oil and its preparations were Bio-control studied using disc-diffusion and Minimum Inhibitory Concentration MIC tests and using corn oil Antimicrobial and Streptomycin as controls. Tolerant ability of EO treated Hep G2 cells was appraised using Cytotoxicity MMT assess and 0.4 0.8 1.2 and 1.6 v v of EO for 3 h were used for flow cytometric explo- Hep G2 ration of cell cycle regression. UPLC MS MS inquiry revealed that EO major ingredients were phe- nyl propanoids followed by monoterpenes hydrocarbons and phenolics and trans-anethole followed by cis-anethole estragole linalool a-terpineol and methyl eugenol were the major EO constituents. EO has high inhibitory effect for Salmonella typhi Enterococcus faecalis Staphylo- coccus aureus Escherichia coli and Micrococcus luteus. Corn oil inhibited E. coli and M. luteus at high concentrations. Streptomycin gave higher inhibition with all bacteria. However both have similar effects with E. faecalis S. typhi and M. luteus bacteria. Spraying potato tubers with EO emulsified with soap prior to Ralstonia solanacearum infection and preservation at 4 C were kept healthy without any rot symptoms. Treating Hep G2 cells with EO for 24 h caused a substantial cytotoxicity as a concentration reliant and exhibited a significant .
