Đang chuẩn bị nút TẢI XUỐNG, xin hãy chờ
Tải xuống
The aim of our in vitro study was to assess the potential effect of BPF on H295R cells mitochondrial activity, metabolic activity, membrane integrity, lysosomal function, and testosterone synthesis. Adrenocortical carcinoma cells were cultivated during 24 h in the presence of BPF (0.1, 0.5, 1, 10, 25, 50, 75, 100, 300, 500 μM). Exposure doses of BPF caused a significant decrease of mitochondrial activity starting from 1 μM, we observed a slight increase in mitochondrial activity at the lowest concentration (0.1 μM). | EVALUATION OF DOSE-DEPENDENT ACTIVITY OF BISPHENOL F ON VIABILITY PARAMETERS AND STEROIDOGENESIS IN H295R CELLS Nikola Knížatová1 Hana Greifová1 Katarína Tokárová1 Tomáš Jambor1 Norbert Lukáč1 Address es 1 Slovak University of Agriculture in Nitra Faculty of Biotechnology and Food Sciences Department of Animal Physiology Tr. A. Hlinku 2 949 76 Nitra Slovak Republic. Corresponding author nikola.knizatova@gmail.com https doi.org 10.15414 jmbfs.4765 ARTICLE INFO ABSTRACT Received 6. 10. 2020 Increasing concern over bisphenol A BPA as an endocrine-disrupting chemical and recent imposition of restriction on the use of BPA paved the way for entry of its analogues in the market. Bisphenol F BPF is one of the major analogues of commercial value. Thus its Revised 16. 4. 2021 increasing production and application make it vulnerable to human exposure. The aim of our in vitro study was to assess the potential Accepted 12. 5. 2021 effect of BPF on H295R cells mitochondrial activity metabolic activity membrane integrity lysosomal function and testosterone Published 1. 10. 2021 synthesis. Adrenocortical carcinoma cells were cultivated during 24 h in the presence of BPF 0.1 0.5 1 10 25 50 75 100 300 500 μM . Exposure doses of BPF caused a significant decrease of mitochondrial activity starting from 1 μM we observed a slight increase in Short communication mitochondrial activity at the lowest concentration 0.1 μM . Metabolic activity decreased with increasing dose of BPF - from 10 to 500 μM. A significant increase in metabolic activity was observed after cultivation with 0.1 μM BPF and a slight increase was observed after cultivation with 0.5 μM BPF. We observed a slight increase in lysosomal function and membrane integrity after cultivation with 0.1 and 1 μM although higher exposure doses 25 - 500 μM caused significant decrease in membrane integrity and lysosomal function. Lowest exposure dose of BPF 0.1 μM caused a significant increase in testosterone synthesis on the other hand
