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Successful application of site-directed mutagenesis polymerase chain reaction to mutate TMS 11 of the staphylococcal multidrug efflux protein QacA

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This research describes the mutation process of the amino acid residues in TMS 11 of QacA using site-direct PCR. In this research, 15 primers were successfully designed for site-directed mutagenesis PCR. The sitemutagenesis PCR was successfully conducted to create 15 qacA mutants. These mutants will be used in further functional research of QacA. |

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Successful application of site-directed mutagenesis polymerase chain reaction to mutate TMS 11 of the staphylococcal multidrug efflux protein QacA

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