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During surveying and collecting of predatory ladybird beetle species in Da Lat city, we recorded that Oenopia kirbyi commonly appeared on aphid-infected plants and weeds, but until now there have been almost no detailed studies on this species. With the hope of contributing to research on the use of predatory ladybird beetles in pest control, we studied the morphological and biological characteristics of Oenopia kirbyi in Da Lat city. | DALAT UNIVERSITY JOURNAL OF SCIENCE Volume 11 Issue 1 2021 14-22 STUDY ON MORPHOLOGICAL AND BIOLOGICAL CHARACTERISTICS OF OENOPIA KIRBYI COLEOPTERA COCCINELLIDAE IN DA LAT CITY LAM DONG VIETNAM Nguyen Thanh Thuy Tiena Nguyen Duc Sanga Le Thi Thanh Thuya Nguyen Thi Thu Huea Nguyen Thi Thuy Ngana Tran Thi Thu Dana a The Faculty of Biology Dalat University Lam Dong Vietnam Corresponding author Email tienntt@dlu.edu.vn Article history Received June 24th 2020 Received in revised form September 17th 2020 Accepted October 14th 2020 Available online February 5th 2021 Abstract Ladybirds are natural enemies of many agricultural crop pests such as aphids scale insects mealybugs whiteflies and thrips. This paper focuses on the morphological and biological characteristics of Oenopia kirbyi Coleoptera Coccinellidae in Da Lat city Lam Dong province . Field-collected adults of Oenopia kirbyi were reared in plastic boxes under laboratory conditions and fed on aphids. The egg phase lasted 4.45 0.71 days the four larval stages lasted 2.63 0.52 days 1.91 0.73 days 1.70 0.46 days and 5.78 0.95 days respectively and pupae lasted 7.02 0.91 days. The total duration of the immature stages was 23.49 days. The adult longevity of males and females was 67.13 18.77 days and 82.83 20.25 days respectively. The fecundity was 414.00 131.33 eggs on average. The rate of hatched eggs emerged adults and the female male ratio were 80.85 96.53 and 1.78 respectively. These results provide basic data for rearing Oenopia kirbyi for use as a biological control of crop pests. Keywords Biological characteristics Morphological characteristics Oenopia kirbyi Predatory ladybird. DOI http dx.doi.org 10.37569 DalatUniversity.11.1.738 2021 Article type peer-reviewed Full-length research article Copyright 2021 The author s . Licensing This article is licensed under a CC BY-NC 4.0 14 DALAT UNIVERSITY JOURNAL OF SCIENCE NATURAL SCIENCES AND TECHNOLOGY 1. INTRODUCTION Da Lat city Lam Dong province is a famous vegetable