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Response surface methodology was applied to optimize the extraction of phenolic compounds from fresh Pouzolzia zeylanica plant using hot water as a solvent. A central composite design (CCD) in form (23+star) was used to investigate the effects of two independent variables, namely, extraction temperature (70 to 90oC) and extraction time (20 to 40 min). | Nong Lam University Ho Chi Minh City 65 Optimization of aqueous extraction conditions for bioactive compounds from fresh Pouzolzia zeylanica plant using response surface methodology Tan D. Nguyen1 2 1 Faculty of Agriculture and Natural Resources An Giang University An Giang Vietnam 2 Vietnam National University Ho Chi Minh City Vietnam ARTICLE INFO ABSTRACT Research Paper Response surface methodology was applied to optimize the extraction of phenolic compounds from fresh Pouzolzia zeylanica plant using hot Received March 02 2020 water as a solvent. A central composite design CCD in form 23 star Revised May 20 2020 was used to investigate the effects of two independent variables namely Accepted June 22 2020 extraction temperature 70 to 90o C and extraction time 20 to 40 min . The dependent variables were the content of anthocyanin flavonoid polyphenol tannin and total soluble solids of extracted solution. A Keywords second-order polynomial model was used for predicting the response. The results showed that the optimal extraction process was obtained Bioactive compounds at 84.4o C for 31.7 min. The experimental values agreed with predicted within a 95 confidence interval. Consequently the contents of antho- Extraction temperature cyanin flavonoid polyphenol and tannin were 38.66 mgCE 100 g 3.01 Extraction time mgQE g 5.17 mgGAE g 4.07 mgTAE g fresh weight and total soluble Pouzolzia zeylanica plant solids content was 0.73 respectively. Response surface methodology Corresponding author Nguyen Duy Tan Email ndtan@agu.edu.vn Cited as Nguyen T. D. 2020 . Optimization of aqueous extraction conditions for bioactive com- pounds from fresh Pouzolzia zeylanica plant using response surface methodology. The Journal of Agriculture and Development 19 3 65-74. 1. Introduction ity at the oral dose of 10 g material powder kg Tran et al. 2010 . Traditionally this plant was Pouzolzia zeylanica is a medicinal source that prepared as an infusion with water to make tea. people of Asia .
