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After 6 months of treatment with antiretroviral drugs based on quantitative HBV-DNA. The rate of normalization of liver enzymes increased significantly. The rate of patients with quantitative HBV-DNA under the detectable level was highly significant. Patients with mutations in the RT region was highly significant (52.9%) while we did not identify any mutations in the Pre-S region. | JOURNAL OF SCIENCE OF HNUE Chemical and Biological Sci. 2014 Vol. 59 No. 9 pp. 139-147 This paper is available online at http stdb.hnue.edu.vn QUANTITATIVE HBV-DNA AND DETERMINATION OF DRUG- RESISTANT MUTANTS IN PATIENTS WITH CHRONIC HEPATITIS B VIRUS HBV IN HAI DUONG PROVINCE Le Thi Phuong Hai Duong Medical Technical University Abstract. The purposes of the paper are i To survey quantitative HBV-DNA in patients with HBsAg Hepatitis B surface antigen positive with antiretroviral drugs and ii To determine drug resistant mutants in the Pre-S and RT regions in patients with quantitative HBV-DNA gt 104 copies mL 6 months after treatment. 195 patients with chronic hepatitis were treated with antiretroviral drugs periodically examined and tested for biochemical indices quantitative HBV-DNA was carried out 6 months before treatment and every 3 months after treatment. After being treated for 6 months patients with quantitative HBV-DNA gt 104 copies mL would be determined to have drug resistant mutants in the RT and Pre-S regions. Group of patients with quantitative HBV-DNA levels gt 106 copies mL decreased from 67.7 pre-treatment to 4.6 after 3 months of treatment and 8.7 after 6 months of treatment group of patients with quantitative HBV DNA positive under detection increased from 0 pre-treatment to 15.9 after 3 months of treatment and 78.5 after 6 months of treatment 17 195 patients with quantitative HBV-DNA decreased but possibly flared up again. These samples carried mutations in the RT and Pre-S regions. The result showed that 9 17 specimens had mutations in the region RT. There were no mutations in Pre-S. Most of the patients were in serious disease condititon before treatment. This resulted in 67.7 of patients with quantitative HBV-DNA gt 106 copies mL. Keywords Quantitative HbeAg HbsAg HBV-DNA real time PCR chronic hepatitis. 1. Introduction The World Health Organization WHO estimates that over 350 and 250 million people worldwide are chronic carrier of hepatitis
