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Identification of selective agents concentrations for optimal plant regeneration from transformed calli and immature embryos in wheat

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Success rate in selection of putative transformants is mainly dependent upon the optimum concentration of selective agent. Effect of antibiotic concentrations of hygromycin (10, 20, 30 and 50 mg/L) and paromomycin (10, 20, 30 and 50 mg/L) in callusing and regeneration media on immature embryo-derived calli was evaluated using wheat variety PBW621. |

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