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The development of nanotechnology interests the researchers for synthesis of nanoparticles with various bio-applications. The green synthesis of copper nanoparticles using Momordica charantia fruit extract acts as both reducing and capping agent. The biosynthesized CuNps were characterized by using UV-Vis analysis, Dynamic Light Scattering (DLS), Fourier Transform Infrared analysis (FTIR), X-ray diffraction analysis (XRD), Scanning Electron Microscopy (SEM) and Transmission Electron Microscopy (TEM) analysis. The synthesized CuNps were in spherical structure with an average size of particle size of 86.07nm. The Antimicrobial activity of the CuNps was established using disc diffusion and agar well diffusion method. The assay showed that CuNps can be a potent antimicrobial agent against Staphylococcus aureus, Pseudomonas aerogenosa and Escherichia coli with no inhibition against Aspergillus flavus. | Green synthesis of copper nanoparticles using Momordica charantia fruit extracts and evaluation of their anti-microbial efficacy