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Microtomy studies of rice tungro disease infected leaves

In our study, cross section of the healthy and Tungro infected leaf sample of rice exhibited wide differences among the cells. The area of vascular bundles was reduced in the Tungro infected rice samples and lower epidermis cells got damaged wherein the area was large in the healthy rice leaf. In case of healthy leaves of rice, the cells were normal in size with properly distributed intercellular spaces and evenly present bundle sheath cells. The xylem and phloem tissues showed no damage in healthy sample. Bundle sheath cells remained undisturbed above the phloem tissues. In the infected rice leaf section, due to the invasion by both the RTBV and RTSV viruses, bundle sheath cells and phloem got distorted. | Int.J.Curr.Microbiol.App.Sci 2019 8 9 1749-1755 EXCELLENT PUBLISHERS International Journal of Current Microbiology and Applied Sciences ISSN 2319-7706 Volume 8 Number 09 2019 Journal homepage http www.ijcmas.com Original Research Article https doi.org 10.20546 ijcmas.2019.809.202 Microtomy Studies of Rice Tungro Disease Infected Leaves P. Valarmathi1 and D. Ladhalakshmi2 1 Department of Plant Pathology ICAR-Central Institute for Cotton Research CICR Coimbatore-641 003 India 2Department of Plant Pathology Department of Plant Pathology ICAR-Indian Institute of Rice Research IIRR Hyderabad-500 030 India Corresponding author ABSTRACT Keywords Rice Tungro disease Microtomy Rice Tungro Bacilliform Virus RTBV Article Info Accepted 20 August 2019 Available Online 10 September 2019 In our study cross section of the healthy and Tungro infected leaf sample of rice exhibited wide differences among the cells. The area of vascular bundles was reduced in the Tungro infected rice samples and lower epidermis cells got damaged wherein the area was large in the healthy rice leaf. In case of healthy leaves of rice the cells were normal in size with properly distributed intercellular spaces and evenly present bundle sheath cells. The xylem and phloem tissues showed no damage in healthy sample. Bundle sheath cells remained undisturbed above the phloem tissues. In the infected rice leaf section due to the invasion by both the RTBV and RTSV viruses bundle sheath cells and phloem got distorted. Introduction Rice Oryza sativa Graminae was domesticated earlier than 5000 B.C. in North East of India. Its cultivation then spread throughout Southeast Asia and Southern China. Rice is the most important staple or subsistence food of world and India. China India Indonesia Bangladesh Vietnam Thailand and Myanmar are the biggest rice producers and account for over 75 per cent of the production Thottapalli 2003 . Annually more than 40 per cent of the world s rice crop is lost owing to .