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Unexpanded leaf rolls were used as explants for callus induction with different concentrations of mutagenic chemicals (sodium nitrite and ethylmethane sulfonate). The effect of these mutagenic chemicals on callusing, shooting and rooting was studied. Callus induction was observed in different concentrations of sodium nitrite (3 mg l-1 , 5 mg l-1 and 7 mg l-1 ) and EMS (0.6 µM, 0.8 µM, 1.0 µM) along with controls. Among all the treatments 3 mg l-1 (T4) SN and 0.6 µM EMS (T10) in 2009T5 were found to be the best for callus induction traits. The treatments responded with minimum number of days for callus initiation, maximum number of explants induced callus with high callus induction frequency and high callus size. | Effects of different mutagenic chemicals on callogensis in sugarcane Saccharum officinarum clones 2008T42 and 2009T5
