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Microbial etiology of duck mortality in Odisha, India

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In the present study 128 samples collected from 66 ducks of various breeds like Batak(28), Moti(34) and Khaki Campbell (6) out of which 112 number of samples from 52 dead birds and 16 number of samples from live birds. blood, liver, swabs from femur bone marrow and also swabs from foot lesions were collected consists of 128 samples in which blood sample (12), swabs from foot lesions(26) of live birds and liver(22), lungs(30) and swab from bone marrow (38) of dead birds. All the samples were processed for routine microbial isolation and identification. Pasteurella species was found to be the most predominant isolate (16%) followed by Pseudomonas species was found to be (11.2%) and E.coli (55%) where as fungal isolates like Aspergillus species was found to be (18%). Pasteurella species were cultivated in 5% sheep blood agar and McConkey’s agar whereas Aspergillus species in SDA by routine inoculation procedure. The ducks were ranging from 2 to 16 weeks of age mostly from unorganised sectors of Odisha. Ducklings were found to be more prone to Pseudomonas and Aspergillus species in comparison to adult ones on antibiotic sensitivity test.it was revealed that tetracycline, doxycycline, oxytetracycline, found to be least sensitive in disk diffusion method. Whereas gentamicin, and ciprofloxacin were found to be highly sensitive against E.coli and gentamicin, amikacin, tobramycin were found to be highly sensitive against Pseudomonas species. Most of the gram negative bacteria isolated in the present study like Pasteurella species, Pseudomonas species and E. coli species were found to be resistant to tetracycline, amphotericin-B. griseofulvin were found to be highly sensitive for Aspergillus species isolated in the present study. | EXCELLENT PUBLISHERS International Journal of Current Microbiology and Applied Sciences ISSN 2319-7706 Volume 8 Number 08 2019 Journal homepage http www.ijcmas.com Original Research Article https doi.org 10.20546 ijcmas.2019.808.186 Microbial Etiology of Duck Mortality in Odisha India Udit Narayan Bariha1 Rajashree Mishra1 AK Kundu2 P.K.Rath3 Chinmoy Mishra4 Sisir Das5 and N. Soren 1 Department of Velerinary Microbiology 2Department of Velerinary Physiology 3 Department of Animal Breeding and Genetics 4Department of Veterinary Physiology College of Veterinary Science and Animal Husbandry OUAT Bhubaneswar India Corresponding author ABSTRACT Keywords Duckmortality Microbial etiology Breeds of duck Article Info Accepted 12 July 2019 Available Online 10 August 2019 In the present study 128 samples collected from 66 ducks of various breeds like Batak 28 Moti 34 and Khaki Campbell 6 out of which 112 number of samples from 52 dead birds and 16 number of samples from live birds. blood liver swabs from femur bone marrow and also swabs from foot lesions were collected consists of 128 samples in which blood sample 12 swabs from foot lesions 26 of live birds and liver 22 lungs 30 and swab from bone marrow 38 of dead birds. All the samples were processed for routine microbial isolation and identification. Pasteurella species was found to be the most predominant isolate 16 followed by Pseudomonas species was found to be 11.2 and E.coli 55 where as fungal isolates like Aspergillus species was found to be 18 . Pasteurella species were cultivated in 5 sheep blood agar and McConkey s agar whereas Aspergillus species in SDA by routine inoculation procedure. The ducks were ranging from 2 to 16 weeks of age mostly from unorganised sectors of Odisha. Ducklings were found to be more prone to Pseudomonas and Aspergillus species in comparison to adult ones on antibiotic sensitivity test.it was revealed that tetracycline doxycycline oxytetracycline found to be least sensitive in disk .

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