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A study was conducted to determine the mycoflora associated with pigeon pea cultivar MA6 and effect of botanicals on growth of mycoflora. The dominant fungi observed on pigeon pea cultivar MA6 seed were Rhizopus stolonifer, Aspergillus niger, A. flavus, Penicillium citrinum, P. rubrum, Alternaria alternata, A. solani, Curvularia lunata, Fusarium oxysporium. Among the treatments, the sulphate and nitrate treated seeds showed the less number of fungi isolated in comparison to untreated seeds. The nature of fungal flora had changed with prolongation of storage period of pigeonpea seeds. Effect of plant botanicals at 15% concentration on the radial growth of mycoflora found that leaf extract of neem (Azadiracta indica) inhibited the growth of Aspergillus niger upto a maximum of 54.85% and Penicillium citrinum was least inhibited. At the same leaf extract concentration of O. sanctum treatment, it was observed to be most inhibitory to Aspergillus flavus which inhibited the fungal growth up to 37.93% and leaf extract of C. longa was observed to be most inhibitory to Rhizopus stolonifer inhibiting the fungal growth up to 31.86%. | Effect of plant botanicals on growth of mycoflora associated with pigeon pea seeds