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Low-cost alternatives for conventional tissue culture media

The low cost alternative components of tissue culture media were evaluated for their potential to supply nutrients and to support growing explant of Banana cultivar in vitro for their cost effectiveness against conventional high cost media. The Murashigeand Skoog (MS) salts and sucrose were replaced by vermicompost (50 g/L), table sugar (30 g/L) and coconut water (70 ml/L), and agar was replaced by filter paper, sand and wheat floor. The conventional MS medium supplemented with 30 g/L sucrose and 8 g/L agar was used as control. | Int.J.Curr.Microbiol.App.Sci 2018 7 4 2523-2529 EXCELLENT PUBLISHERS International Journal of Current Microbiology and Applied Sciences ISSN 2319-7706 Volume 7 Number 04 2018 Journal homepage http www.ijcmas.com Original Research Article https doi.org 10.20546 ijcmas.2018.704.288 Low-Cost Alternatives for Conventional Tissue Culture Media Dipak D. Kadam1 Amit A. Chhatre1 Shivaji A. Lavale2 and Nalini A. Shinde1 1Lokmangal College of Agricultural Biotechnology Solapur MS India Inlernalional Crops Research Institute for Semi-Arid Tropics Hyderabad India Corresponding author ABSTRACT Keywords Low cost medium MS medium Micropropagation Tissue culture Cost effectiveness Article Info Accepted 20 March 2018 Available Online 10 April 2018 The low cost alternative components of tissue culture media were evaluated for their potential to supply nutrients and to support growing explant of Banana cultivar in vitro for their cost effectiveness against conventional high cost media. The Murashigeand Skoog MS salts and sucrose were replaced by vermicompost 50 g L table sugar 30 g L and coconut water 70 ml L and agar was replaced by filter paper sand and wheat floor. The conventional MS medium supplemented with 30 g L sucrose and 8 g L agar was used as control. A banana cultivar was regenerated on control and combinations of alternative components. MS salts in combination with filter paper as solid matrix was found as very efficient for shoot induction multiplication and root formation with reducing the cost upto 58.13 .Vermicompost as nutritive source was found to be very effective in reducing cost of media per plantlet. Table sugar reduced the cost of carbon source drastically by 97 .Use of such alternatives can reduce the cost of media thereby minimizing the production cost of tissue cultured plantlets. Introduction The demand for high-quality high-yielding disease-free planting material has increased significantly over the last two decades with increasing demand for agricultural