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The present study was conducted on estimation of biochemical parameters i.e. sugar content, gossypol content, protein content and oil content in 3 genotypes of Upland cotton grown in six environments. The results showed that The early sown condition was best for high seed germination (%), high sugar (%), protein (%), oil (%) and low gossypol (%). Early sown conditions were good for desirable biochemical parameters. Protein (%) was positively correlated with seed germination (%), whereas it was negatively correlated with sugar. | Estimation of biochemical parameters in different environments in upland cotton (Gossypium hirsutum L.)