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Field trial was conducted for two consecutive years during kharif 2017 and 2018 at farmer`s field, Kadarmandalagi by using randomized block design (RBD). During kharif 2017, among the 20 genotypes screened indicated that lowest mean population of yellow mite and highest mean leaf hair density and trichome length were recorded in Chaitanya (MRC-7377) (0.29 yellow mite/ leaf) followed by Soumya (SRCH-55) and were superior to all the genotypes. While highest mean yellow mite population and least mean leaf hair density and trichome length were observed in Super fibre (KDCHB-407) (2.96 yellow mite/ leaf). During kharif 2018, two resistant and two susceptible genotypes were chosen based on performance during kharif 2017 for biochemical cgaracter analysis. Morphological characters like trichomes and trichome length and biochemical character phenol were recorded highest in resistant genotypes Chaitanya (MRC-7377) and Soumya (SRCH-55). While, susceptible genotypes Super fibre (KDCHB-407) and Surya (Namkot-803) were recorded lowest trichomes and trichome length and highest sugars and proteins. | Screening of different Bt cotton genotypes against yellow mite, Polyphagotarsonemus latus (Banks) and biochemical basis of resistance