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A study was conducted with nine gladiolus genotypes namely Arka Aayush, Arka Amar, Arka Darshan, Arka Gold, Arka Kumkum, Pink Friendship, IIHRG-12, Gladiolus callianthus and Psittacinus hybrid for resistance against two geographically different isolates of Fusarium oxysporum f. sp. gladioli (FOG) i.e. FGS-SOL isolate and FOG IIHR-1 isolate. Pink Friendship recorded highest disease incidence (91.67 %) which was inoculated by FOG IIHR-1 isolate whereas no disease incidence was observed in inoculated plants of Psittacinus hybrid. The dendrogram deduced from various characters showed high similarity between Arka Aayush and Arka Darshan in FGS-SOL isolate inoculation and between Arka Amar and Arka Kumkum in FOG IIHR-1 isolate inoculation. Disease progress was measured through area under disease progression curve (AUDPC) and highest AUDPC value was recorded in Pink Friendship inoculated with FOG IIHR-1 isolate. Genotypes Arka Amar, Pink Friendship and IIHRG-12 took minimum days to sprouting, spike emergence and opening of 1st floret, respectively. Gladiolus callianthus exhibited maximum plant height and spike length. Number of corms per corm was highest in Psittacinus hybrid. Arka Gold recorded maximum average weight of corm and diameter of corm. Resistant genotypes which were identified can be used in breeding programs for development of resistant cultivars. | Evaluation of Gladiolus genotypes for Resistance to different isolates of fusarium oxysporum f. sp. gladioli