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Despite Fowl typhoid (FT) eradication in commercial poultry of some parts of the world, outbreak threats are not eliminated in poultry of developing countries. In the current study, we reported molecular identification of Salmonella gallinarum in Indian isolates from backyard poultry by a multiplex PCR assay. Boiled culture lysates of Salmonella Gallinarum isolates (n=2) from an outbreak showed positive amplifications of glgC and speC genes in duplex PCR with 174 bp and 252 bp products, respectively. In multiplex PCR assay, primers for salmonella plasmid virulence gene (spvC) were also added in same reaction mixture. No amplifications were noticed in negative controls with other Salmonella serotypes and non –Salmonella organisms. This multiplex PCR assay was found to be rapid and specific in diagnosis of fowl typhoid. | Evaluation of a multiplex PCR assay for rapid diagnosis of fowl typhoid
