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This investigation was carried out to study combining ability along with inheritance of grain yield and its component traits in 100 hybrids of pearl millet which were generated through line x tester mating design using 5 male sterile lines and 20 restorers as parental material at ICRISAT, Hyderabad during Summer, 2018. These hybrids were evaluated in randomized block design with 3 replications in 3 environments during Kharif, 2018 at Rajasthan Agricultural Research Institute, Durgapura (Jaipur). In results, the ratio of variance due to GCA and SCA indicated that predominance of non-additive gene action for all the characters studied except ear girth. GCA effects revealed that parents like ICMA 04999 and ICMA-843-22 (female), RIB-192, RIB-15270, RIB-494 and MIR-525-2 (male) were good general combiners for grain yield and some contributing characters. On the basis of SCA effects the crosses namely ICMA-843-22 x RIB-3135-18, ICMA-04999 x J-2290, ICMA-93333 x RIB-15270 were identified as superior for grain yield and related traits over the. | Combining ability and gene action for grain yield and related components in pearl Millet [Pennisetum glaucum (L.) R.Br.]