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The role of testosterone in reproductive performance of Amur common carp has been studied during different seasons using the RP-HPLC method from gonad and plasma of two different age groups of both sexes. Gonadal and plasma T levels in both the age groups showed similar seasonal trend with highest level in spring season, which decreased in summer and continued to decrease to the lowest level in autumn followed by slight increase in winter season. Statistically significant differences (p | Seasonal changes in the testosterone level in different age groups of amur common carp