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Daffodil (Narcissus spp.) belongs to family Amaryllidaceae and is a bulbous perennial grown for attractive flower, borne in spring sometimes autumn or winter. The present study was carried out to assess the genetic diversity present in daffodils in temperate region of Kashmir India using eleven sample sequence repeats (SSR’s). Twenty seven genotypes of daffodils belonging to different species such as Narcissus incomparabilis, Narcissus pseudonarcissus, Narcissus jonquilla, Narcissus poeticus, Narcissus papyraceus were evaluated for molecular characterization. Microsatellite markers revealed a high level of polymorphism and Jaccord’s Similarity coefficient ranged from 0.05 to 0.98. Analysis of molecular variance (AMOVA) revealed high level of variability of 94.89 per cent within population. Whereas among population variability is 5.11 per cent. The expected heterozygosity was shown highest (0.73) by marker A131 in Narcissus incomparibilis and total heterozygosity across different specieswas shown highest (0.71) by marker A5. The mean expected heterozygosity was shown highest (0.43) by Narcissus incomparabilis followed by Narcissus pseudonarcissus which revealed mean expected hytrozygosity of 0.37. The locus A109 and B112 revealed highest effective alleles of 10 each, followed by A5 and B104 recording effective alleles of 9 and 8 respectively. | Genetic diversity analysis based on SSR markers in daffodils (Narcissus)
