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Fish acts as an intermediate host to many helminth parasites, including trematodes of public health significance. In this study, we screened freshwater fish samples (n=195) collected from different markets located in and around Kolkata, to isolate and identify larval stage of minute intestinal fluke, Centrocestus formosanus employing a fast and accurate molecular approach. | A report of centrocestus formosanus Nishigori 1924 Digenea Heterophyidae in intermediate host Fish