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100 genotypes from germplasm were taken to evaluate oil content, total glucosinolate content, erucic acid, fibre content, phenol and sinigrin. The assayed genotypes contained 38.38- 42.89 % oil, 38.97-113.01 µmole/g glucosinolate, 18.67-47.05 % erucic acid, 1.03-1.93% Phenols, 7.82- 14.58% fibre and sinigrin 9.40-107.34% content using FT-NIR. The objective of this study is to characterize the large population of genotypes with advanced technique e.g. FT-NIR within short time period. Result clearly showed that seeds of core set of Indian mustard having high glucosinolates, grown at a field site revealed a wide variation in total concentrations of seed oil, erucic acid, phenols and mainly sinigrin. | Evaluation of Indian mustard for their potential nutritional and antinutritional factors