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The experiment was conducted in Punjab Agricultural University, Ludhiana, Punjab during the season of 2016 and 2017, in order to determine the pollen viability response to storage method and period in Gladiolus (Gladiolus grandiflorus L.). Gladiolus var. Yellow stone used as male parent for pollen collection. The corms of gladiolus were planted during October–December to obtained fresh pollen for research. Pollen grains of variety Yellow stone packed in plastic vials were stored in desiccator at ambient temperature, desiccator with CaCl2 at ambient temperature, desiccator under refrigerator, desiccator with CaCl2 under refrigerator (5˚C), lower cabin under refrigerator (5˚C), freezer(-4°C) for 16 weeks from January to April. Pollen stored under different storage methods was used for determination of pollen viability. Maximum pollen viability was recorded under freezer i.e. 82.87%. Pollen stored under freezer retained pollen viability for longer duration and it was recorded 85.09% after 11weeks and pollen viability decreases to 49.58% after 16 weeks of storage. Pollen of the gladiolus can be stored for longer duration under freezer condition up to 11 weeks of storage without any serious loss of viability. The pollen of gladiolus can be stored in freezer (-4˚C) up to 2½ month for obtaining good pollen viability. | Response of pollen viability in Gladiolus (Gladiolus grandiflorus L.) to storage method and period
