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Toxoplsmosis is a disease of all regions and communities. The present study was conducted in 2017 to evaluate Toxoplasma prevalence and related knowledge in pregnant women and university staff of Kirkuk city.1000 individuals had submitted a questionnaire form to assess their knowledge on Toxoplasma. Sero-prevalence screen of the disease was done by ELIZA technique in 276 individuals of the pregnant women. The total infection rate of the disease among the pregnant women was 9.8%. Entirely the educated persons had more knowledge background on toxoplasmosis comparing to uneducated, in both university staff and pregnant women. The proportion of university staff who knew what is the disease was 41.4 %. While a proportion of 58.6% did not knew the disease. Among pregnant women a proportion of 35.3% had knew some information on the disease and a rate of 64.7% of them were only heard on the disease. Very high percentages of questioned cohorts have no information on the diseases host types, modes of transmission, infected body organs, populations more at risk for getting the infection and other diseases related knowledge's because the disease is more related to women than men. These results highlight the significance of a regular seminars and conferences on toxoplasmosis among females especially pregnant, as well as the importance of periodically serological screening process during prenatal care. | Toxoplasma sero-prevalence and related knowledge survey in pregnant women and university staff