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The yearlings of Rohu (Labeo rohita) was fed with commercial pellated feed as T1(Control), feed incorporated with Lactobacillus sporogenes @ 4% as T2, Saccharomyces cerevisiae @ 4% as T3 and both Lactobacillus sporogenes @2% and Saccharomyces cerevisiae @ 2% as T4.The experiment was designed for 120 days in the cement tanks. Feeding was done with probiotics and without probiotics at alternate 15 days. Sampling was done at an interval of 15 days. The samples were analysed to determine the weight gain %, specific growth rate %, FCR, FER and TPC of probiotic microbes. | Effect of feed probiotic on the growth and their colonization performance on the intestine of rohu (Labeo rohita) Int.J.Curr.Microbiol.App.Sci (2020) 9(3): 806-823 International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 9 Number 3 (2020) Journal homepage: http://www.ijcmas.com Original Research Article https://doi.org/10.20546/ijcmas.2020.903.095 Effect of Feed Probiotic on the Growth and their Colonization Performance on the Intestine of Rohu (Labeo rohita) Nityananda Das1*, Sarita Das*, B. K. Khuntia and Brundaban Sahu College of Fisheries (OUAT), Rangailunda, Berhampur-7, Ganjam, Odisha, India *Corresponding author ABSTRACT The yearlings of Rohu (Labeo rohita) was fed with commercial pellated feed as T1(Control), feed incorporated with Lactobacillus sporogenes @ 4% as T2, Saccharomyces cerevisiae @ 4% as T3 and both Lactobacillus sporogenes @2% and Saccharomyces cerevisiae @ 2% as T4.The experiment was designed for 120 days in the cement tanks. Keywords Feeding was done with probiotics and without probiotics at alternate 15 days. Sampling was done at an interval of 15 days. The samples were analysed to determine the weight Probiotic, Feed, gain %, specific growth rate %, FCR, FER and TPC of probiotic microbes. The average Growth, Rohu initial weight of fish in all treatment was about 44 g. After feeding with probiotic incorporated feed, the weight increased to 150.78±0.68 gm, 176.13±0.75g and 183±0.91g Article Info in T2, T3 and T4 respectively as against 102.05±0.99g in T1(control). After first 15 days Accepted: there were probiotic bacteria in all treatments except control. After next 15 days of feeding 05 February 2020 without probiotics, in all treatments (i.e. in 30 days) the TPC of probiotic microbe was Available Online: found to be 0 in both T 1 and T2 except T3 and T4. Likewise after 120 days the TPC of 10 March 2020 probiotic microbe in T 1and T2 was .